Red Florescent Protein Lab
Purpose:
Make RFP from jelly fish in bacteria and to learn more about steps in genetic engineering.
Materials/Procedure:
2a- materials and procedure can be found in Amgen lab manual part 2a
4a- materials and procedure can be found in Amgen lab manual part 4a
5a- materials and procedure can be found in Amgen lab manual part 5a
6a- materials and procedure can be found in Amgen lab manual part 6a
Experimental Overview:
Part 2a: Verification of plasmid by restriction digest. We cut the plasmid with BamH1 and Hind III to cut out RFP-ara from bacteria plasmid.
Part 4a: Verification of plasmid digest by electrophonesis. We used electrophonesis to make sure we correctly cut the plasmid. We ran it in a gel with a DNA ladder.
Part 5a: Transformation of bacteria with recombinant plasmid. We changed teh bacteria using a recombinant plasmid. Using restiction enzymes, we took the gene we wanted, and put it with the bacteria.
Part 6a: Verification RFP using chromatography. We used a column with beads to collect the RFP and separate the RFP with the other liquid.
Results:
Make RFP from jelly fish in bacteria and to learn more about steps in genetic engineering.
Materials/Procedure:
2a- materials and procedure can be found in Amgen lab manual part 2a
4a- materials and procedure can be found in Amgen lab manual part 4a
5a- materials and procedure can be found in Amgen lab manual part 5a
6a- materials and procedure can be found in Amgen lab manual part 6a
Experimental Overview:
Part 2a: Verification of plasmid by restriction digest. We cut the plasmid with BamH1 and Hind III to cut out RFP-ara from bacteria plasmid.
Part 4a: Verification of plasmid digest by electrophonesis. We used electrophonesis to make sure we correctly cut the plasmid. We ran it in a gel with a DNA ladder.
Part 5a: Transformation of bacteria with recombinant plasmid. We changed teh bacteria using a recombinant plasmid. Using restiction enzymes, we took the gene we wanted, and put it with the bacteria.
Part 6a: Verification RFP using chromatography. We used a column with beads to collect the RFP and separate the RFP with the other liquid.
Results: